genomics of salt tolerance
Stress genomics of salt tolerance
Salicornia brachiata Roxb. (Amaranthaceae), a succulent annual halophyte, which can tolerate salinity upto 2.0 M NaCl was selected as a model plant for stress genomic study. Different abiotic responsive responsive genes viz. SbMT-2, SbpAPX, SbGST-1, SbNHX-1, SbCLC, SbUSP, SbSR-1 and SbSR-2 were isolated from the halophyte and functionally validated using transgenic approaches.
Additionally, promoters of salt responsive genes were also characterized and transcriptional regulation of genes are now targeted for the further studies.
India is one of the mega biodiversity countries in the world. There is an awakening all over the world on the use of age old wisdom of traditional systems of medicine to combat diseases of multiple natures and utilize the wealth of flora, particularly medicinal herbs, used in these systems. This awakening has created an increased interest among the pharmaceutical industry and others on the use of medicinal plants.
Considering the fact that herbal industry is growing rapidly in India, there is a great demand for supply of consistent quality raw material/bio-active molecules of medicinal plants for their utilization in drugs and pharmaceuticals, nutraceuticals and cosmaceuticals. Therefore, there is an urgent need to bioprospect the plant diversity and to document the genetic diversity at inter-specific and inter-population across different phytogeographic zones by using chemical and molecular markers.
Using different analytical tools and techniques like GC-MS, LC-MS, MALDI-TOF-TOF etc. we are targeting metabolites from the halophyte S. brachiata as well as glycopgytes Cumin (Cuminum cyminum) and Isabgul (Plantago ovata).
We have studied the abundance of functional genes and bacterial community structure from coastal area of Gujarat by gene targeted metagenomics approach using different functional gens like cbbL, nifH, amoA and apsA and real time PCR. Molecular phylogeny showed a significant variation in the bacterial community distribution along the Gujart coast. A high number of novel taxonomic units were also observed which makes the entire area a unique reservoir of unidentified bacterial taxa that may be explored further.
Exploration of Marine Flora for Extra-cellular
Polymeric Substances [EPSs]
Exploration of Marine Flora for Extra-cellular Polymeric Substances [EPSs]
Marine environment provides a suitable niche for EPSs producing bacteria/ micro-algae. The EPS secreted by marine micro-organism forms the main constituent of biofilm. It’s structural and functional diversity is an important attribute for potential biotechnological and industrial applications. In recent years lot of attention has been paid to microbial EPSs.